The Africa Centre has, over the last decade or so, built up an extensive collection of biological specimens of various kinds. These are mostly collected from the population around the Africa Centre in northern KwaZulu-Natal, but there are also specimens collected from populations in and around Durban and elsewhere in KwaZulu-Natal. The results of tests carried out on these specimens are generally stored in the main databases of the various studies involved, and are linkable back to the demographic and other data collected from the individuals concerned.
The Biobank is curated by staff of the Africa Centre’s Virology Laboratory in Durban, where all the specimens are currently stored, mostly in -80C freezers. A particular strength of its holdings are the dried blood spot (DBS), specimens – five drops of blood on a filter-paper card, obtained via a finger-prick - of which there are now nearly 115,000.
The following is a list of its holdings as at May 2011.
· 67,700 DBS specimens collected since late 2002 primarily for HIV prevalence estimation of the population covered by the Africa Centre Demographic Surveillance population. All have at least been tested for HIV, and just over 21% give a Positive result. Specimens are collected annually, so for some individuals we might have a sequence of 8-10 specimens covering 2002-2011.
· 36,601 DBS specimens collected by the Vertical Transmission Study (VTS) between Sep 2001 and Dec 2006. This study focussed on mother-child pairs and investigated the vertical transmission of HIV from mother to child. DBS specimens were collected from both the mothers (at initial screening, and then from their children at Birth, 6, 10, 14, 18, 22 weeks, and 7, 8, 9, 12, 15, 18, 21 and 24 months.
· 6,585 DBS specimens collected as part of the KZN IMPACT study of PMTCT effectiveness in six districts of kwaZulu-Natal. The specimens were collected during 2004-2006 from infants aged 4-8 weeks when mothers brought them to clinics for immunisation. These DBS specimens are stored at room temperature, not in freezers.
· 3,524 DBS specimens collected as part of the Kesho Bora study from Sep 2007 . They were collected from mothers at enrolment, and from the infants at delivery, 2 weeks, and at 4, 5, 7, 8 and 15 months.
· 50,068 Plasma specimens comprised of
o 20,096 from the Vertical Transmission Study
o 11,314 from Kesho Bora
o 9,266 from Africa Centre DSS HIV Surveillance
o 2,677 from the MDP Microbicide Feasibility Study
o 1,598 from McCords Hospital Study Group (Resistance testing)
o 1,541 from the KST Study (Kaposi’s Sarcoma)
o 1,314 from the Micronutrients (MICS) study
o 1,113 from the ANC/ANS study in 2005 (Antenatal HIV surveillance)
o 906 from the Pediatric ARV Study (“PARV”)
o 687 from the AMNS Maternal Nutrition Study
o 556 from MTCT
· 28,775 breastmilk specimens
o 27,349 from the Vertical Transmission Study
o 1,426 breastmilk cells from Kesho Bora
o 5,033 breastmilk Lactoserum from Kesho Bora
· 11,277 breastmilk products (Pellets and lactoserum). These have all been extracted from the BM specimens in prev. item?
· 11,188 RNA and DNA products extracted from DBS and plasma specimens from all our major studies.
· 5,735 Serum specimens made up of:
o 3,990 from Africa Centre DSS HIV Surveillance
o 1,701 from Kesho Bora
o 44 from MTCT PLUS
· 3,505 cell pellets made up of:
o 1,972 from the Vertical Transmission Study (VTS)
o 585 from MTCT PLUS
o 442 from the “AMNS” Maternal Nutrition Study
o 213 from Pediatric ARV Study
o 190 from McCords Hospital Study Group (Resistance testing)
o 95 from the KST study (Kaposi’s Sarcoma)
o 8 from Micronutrients Study
· 1,778 whole blood specimens comprised of:
o 1,216 from the Vertical Transmission Study (VTS)
o 305 from the “AMNS” Maternal Nutrition Study
o 166 from Kesho Bora
o 87 from MTCT PLUS (?)
o 4 from McCords Hospital Study Group (Resistance testing)
· 1,284 Peripheral Blood Mononuclear Cells from the Kesho Bora study mothers (665) and their children (619)
· 179 skin tissue specimens from the KST study (Kaposi’s Sarcoma)
· 176 foreskins, ~130 of which were collected during a Foreskin study (?), and the rest as part of a Maternal Nutrition Study (“AMNS”)